منابع مشابه
Pectic enzymes in pectolyase: separation, characterization, and induction of ethylene in fruits.
The pectic enzymes in Pectolyase were separated by ion exchange chromatography on Q-Sepharose. Three pectin lyases, two polygalacturonases, and a pectinmethylesterase were resolved. The enzymes were further purified on Mono Q and/or Mono S columns to remove traces of cellulase. The enzymes had molecular weights ranging from 25,000 to 36,000 daltons. They were optimally active between pH 4.0 and...
متن کاملPectic Enzymes
Indoleacetic acid (IAA) and/or inhibitors of DNA, RNA or protein synthesis were added to the apex of decapitated seedlings of Pisum sativum L. var. Alaska. At various times up to 4 days, enzymic protein was extraoted from a segment of epicotyl immediately below the apex and assayed for its ability to hydrolyse polysaccharides or their derivatives. With the exception of am;ylase, the total amoun...
متن کاملProduction of pectic enzymes in yeasts.
When grown in the appropriate medium, several yeast species produce pectinases able to degrade pectic substances. It is mainly exocellular endopolygalacturonases that break pectins or pectate down by hydrolysis of alpha-1,4-glycosidic linkages in a random way. Biochemical characterisation of these enzymes has shown that they have an optimal pH in the acidic region and an optimal temperature bet...
متن کاملRelationship of Pectic Enzymes to Abscission
A comprehensive review of work pertinent to changes in the separation zone prior to abscission is presented. Since these changes are largely pectic in nature the involvement of pectic enzymes is suggested. The results obtained for the role of pectin methylesterase ( PME) in abscission arc as follows: ( 1 ) A localization of PME activity in the abscission zone of tobacco pedicels was established...
متن کاملMicrobial Pectic Enzymes in the Food and Wine Industry
Pectins are polysaccharides ubiquitous in the plant kingdom and constitute the major component of plant cell walls. The pectinases are a group of related enzymes capable of degrading pectin. Therefore, this group of enzymes have been used for decades in the food and winemaking industry for the processing of fruit juices (Mohnen, 2008; Prade et al., 1999; Ribeiro et al., 2010). The pectinases ar...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1989
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.90.1.191